Fig. 2
From: The crucial role of Erk2 in demyelinating inflammation in the central nervous system
Deletion of Erk2 in neural cell population. a Immunohistochemical staining of samples of cortex taken from adult Nestin-Cretg: CAG-floxed Neo-EGFP mice confirms that recombination occurs in neurons (NeuN+) and astrocytes (GFAP+) (a, arrowhead) but not in microglia (Iba-1+). b, c Nestin-Cretg: Erk2flox/flox (Erk2 cKO) mice treated with cuprizone. Western blotting of phospho-Erk shows selective deletion of Erk2 (b). Immunoreactivity of phospho-Erk is also reduced in corpus callosum in cuprizone-treated Erk2 cKO mice (c). Note that antibody against phospho-Erk reacts with both phospho-Erk1 and phospho-Erk2. The lines in c indicate the boundary of the corpus callosum. Scale bars: a, 50 μm; c, 100 μm