Table 1 Primers used for RT-PCR
From: The crucial role of Erk2 in demyelinating inflammation in the central nervous system
TNF-alpha (mouse) | Sense | 5′-ACGGCATGGATCTCAAAGAC-3′ |
Anti-sense | 5′-GTGGGTGAGGAGCACGTAGT-3′ | |
IL-1beta (mouse) | Sense | 5′-CAGGCAGGCAGTATCACTCA-3′ |
Anti-sense | 5′-ATGAGTCACAGAGGATGGGC-3′ | |
Ccl-2 (mouse) | Sense | 5′-CCCAATGAGTAGGCTGGAGA-3′ |
Anti-sense | 5′-TCTGGACCCATTCCTTCTTG-3′ | |
Ccl-3 (mouse) | Sense | 5′-ATGAAGGTCTCCACCACTGC-3′ |
Anti-sense | 5′-GATGAATTGGCGTGGAATCT-3′ | |
Ccl-5 (mouse) | Sense | 5′-GTGCCCACGTCAAGGAGTAT -3′ |
Anti-sense | 5′-CACTTCTTCTCTGGGTTGGC-3′ | |
Cxcl-10 (mouse) | Sense | 5′-AAGTGCTGCCGTCATTTTCT-3′ |
Anti-sense | 5′-CAATGATCTCAACACGTGGG-3′ | |
MBP (mouse) | Sense | 5′-ACTCACACACGAGAACTACCCA-3′ |
Anti-sense | 5′-TGGTGTTCGAGGTGTCACAA-3′ | |
MAG (mouse) | Sense | 5′-GGTACATGGCGTCTGGTATTTCA-3′ |
Anti-sense | 5′-CCACTTGTGTGCGGGACTT-3′ | |
TNF-alpha (rat) | Sense | 5′-ACGGCATGGATCTCAAAGAC-3′ |
Anti-sense | 5′-GTGGGTGAGGAGCACGTAGT-3′ | |
IL-1beta (rat) | Sense | 5′-AAAAATGCCTCGTGCTGTCT-3′ |
Anti-sense | 5′-GGGATTTTGTCGTTGCTTGT-3′ | |
Ccl-2 (rat) | Sense | 5′-ATGCAGTTAATGCCCCACTC-3′ |
Anti-sense | 5′-TTCCTTATTGGGGTCAGCAC-3′ | |
Ccl-3 (rat) | Sense | 5′-CTTCTCCTATGGACGGCAAA-3′ |
Anti-sense | 5′-CGGTTTCTCTTGGTCAGGAA-3′ | |
Ccl-5 (rat) | Sense | 5′-ATATGGCTCGGACACCACTC-3′ |
Anti-sense | 5′-TGACAAAGACGACTGCAAGG-3′ | |
Cxcl-10 (rat) | Sense | 5′-CTTCCATGAACAGACGCTGA-3′ |
Anti-sense | 5′-TCTTGATGGCCTCAGATTCC-3′ | |
GAPDH (rodent) | Sense | 5′-TGCACCACCAACTGCTTAGC-3′ |
Anti-sense | 5′-GGATGCAGGGATGATGTTCT-3′ |