Fig. 1

β-Catenin positively regulates EAAT2 mRNA and protein expression in PDAs. PDAs were transfected with siRNA for β-catenin or scrambled siRNA (Scrm-si), and 72 h post transfection, total β-catenin protein was monitored by WB (a), EAAT2 mRNA level was monitored by real-time qPCR (b), and EAAT2 protein expression monitored by WB (c). PDAs were transfected with a plasmid containing a constitutively active form of β-catenin (pABC) or a control plasmid and 24 h later transfected with a TCF/LEF reporter plasmid (TOPflash) and luciferase activity was measured 24 h later (d). PDAs were transfected with pABC or control vector (pcDNA) (e, f), and 24 h post transfection, EAAT2 mRNA (e) and protein (f) levels were monitored by qPCR and WB, respectively. Asterisks indicate a p < 0.05 relative to the respective control using Student’s t test. Error bars indicate SD