Fig. 4

Sub-acute Prussian blue-positive cerebral microhemorrhages: Significantly higher number of PB-positive stains in LPS (1 mg/kg, at 0, 6, and 24 h; n=9)- compared with saline-treated (n=9) mice (comparisons shown in black) (a). Differences in regional distribution of PB-positive stains in the cortex, sub-cortex, and cerebellum of LPS- and saline-treated mice (comparisons shown in blue) (a). Representative images showing PB-positive stains in different brain regions (b). Scale bar = 100 μm. Significantly higher PB-positive area in the LPS- compared with saline-treated mice (c). Data are presented as mean ± SEM. Mann-Whitney U test to compare two groups and two-way repeated measure ANOVA with Bonferroni’s post-test for more than two groups; *p < 0.05, **p < 0.01, ****p < 0.0001