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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Cerebral ischemia-induced angiogenesis is dependent on tumor necrosis factor receptor 1-mediated upregulation of α5β1 and αVβ3 integrins

Fig. 7

TNF-α-induced HBMEC proliferation was abrogated by antibody neutralization of the α5 or αVβ3 integrins. CFSE-labeled HBMECs were pre-incubated with neutralizing antibodies (5 μg/ml) against the human integrin α5 or αVβ3 integrins, or a combination of both for 30 min and then stimulated with or without 10 ng/ml rhTNF-α for an additional 24 h before being harvested and analyzed for cell proliferation by flow cytometry. a Histogram of HBMEC proliferation with indicated treatments analyzed by ModFit software. b Quantification of HBMEC proliferation. The proliferation index was quantified using ModFit software and expressed as the percentage of the control. The results represent the mean ± SEM of four experiments. Note that TNF-α significantly increased HBMEC proliferation relative to control conditions (**P < 0.01); and this was abrogated by either anti-α5, anti-αVβ3, or a combination of both antibodies. #P < 0.05, ##P < 0.01 versus positive control

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