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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Astroglia acquires a toxic neuroinflammatory role in response to the cerebrospinal fluid from amyotrophic lateral sclerosis patients

Fig. 5

ALS-CSF conditioned astroglial medium imparts neurotoxicity to NSC-34 cells (a–c). The phase contrast images of NSC-34 cells depicting the effect of the conditioned media from astroglial cultures exposed to NALS/ALS-CSF (NALS/ALS-ACM) or NC (NC-ACM). Note the extensive vacuolation (c, arrowhead) and dying cells (c, arrow) in ALS-ACM subsets. The corresponding graph (graph d) shows a significant reduction in the percent cell viability as measured by altered pattern of MTT reduction, in the NSC-34 cells exposed to ALS-ACM as compared to the NC-ACM or NALS-ACM groups (***p < 0.001 NC v/s ALS; n = 5 in triplicates). Analysis of significance was carried out using one-way ANOVA followed by Tukey’s post hoc test. Scale bar is indicated

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