Table 2 List of the antibodies used in the study
Primary antibody | Secondary antibody |
|---|---|
Anti-COX-2 rabbit polyclonal (1:500, Abcam); 24 h, 4 °C | Anti-rabbit IgG (Cy3-conjugated; 1:200, Sigma-Aldrich) 2 h, RT |
Anti-GDNF mouse monoclonal (1:200, SCBT), 24 h, 4 °C | Anti-mouse IgG (Cy3-conjugated; 1:200, Sigma-Aldrich) 2 h, RT |
Anti-IFN-γ goat polyclonal (1:200, SCBT) 24 h, 4 °C | Anti-goat IgG (Cy3-conjugated; 1:200, Sigma-Aldrich) 2 h, RT |
Anti-IL-10 rabbit polyclonal (1:500, Abcam) 24 h, 4 °C | Anti-rabbit IgG (Cy3-conjugated; 1:200, Sigma-Aldrich) 2 h, RT |
Anti-IL-6 rabbit polyclonal (1:500, Abcam) 24 h, 4 °C | Anti-rabbit IgG (FITC-conjugated; 1:200, Chemicon) 2 h, RT |
Anti-iNOS rabbit polyclonal (1:800, Abcam) 24 h, 4 °C | Anti-rabbit IgG (Cy3-conjugated; 1:200, Chemicon) 2 h, RT |
Anti-PGE-2 rabbit polyclonal (1:500, Abcam) 24 h, 4 °C | Anti-rabbit IgG (FITC-conjugated; 1:200, Chemicon) 2 h, RT |
Anti-TNF-α mouse monoclonal (1:200, Abcam), 24 h, 4 °C | Anti-mouse IgG (Cy3-conjugated; 1:200, Sigma-Aldrich) 2 h, RT |
Anti-VEGF rabbit polyclonal (1:500 Abcam), 24 h, 4 °C | Anti-rabbit IgG (FITC-conjugated; 1:200, Chemicon) 2 h, RT |