Fig. 10
TCLPA5 suppresses ROS and NO production. a The intracellular ROS levels generated by BV-2 and PMM were determined using carboxy-H2DCFDA. Serum-starved BV-2 and PMM were incubated with carboxy-H2DCFDA, treated with vehicle control, LPA (1 μM), or LPA plus TCLPA5 (5 μM) for the indicated time periods, and the fluorescence intensity was evaluated. Results (three independent experiments performed in triplicate) are presented as mean values + SD. (**p < 0.01; ***p < 0.001 compared to vehicle; # p < 0.05 compared to LPA treated cells; one-way ANOVA with Bonferroni correction). b Serum-starved BV-2 and PMM were incubated with vehicle (DMSO), LPA (1 μM), or LPA plus TCLPA5 (5 μM) for the indicated times, and the production of NO was determined by measuring the total nitrate concentration in the supernatants. Data (three independent experiments performed in triplicate) are presented as mean values + SD. (*p < 0.05; compared to untreated cells; # p < 0.05 compared to LPA treated cells; one-way ANOVA with Bonferroni correction)