Fig. 2

LPA alters microglia morphology. Staining for F-actin and β-tubulin in a BV-2 cells and b primary murine microglia (PMM). Cells on chamber slides were serum starved (o/n) and incubated in the absence or presence of LPA (1 μM, 24 h). After washing and incubation with primary and secondary antibodies, cells were analyzed by confocal microscopy. Nuclei were stained with DAPI. Representative images reveal rearrangement of the cytoskeleton following LPA treatment. Scale bar = 20 μm. Results from one representative experiment (out of two) are shown