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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: 1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 and primary murine microglia towards an M1-like phenotype

Fig. 6

LPA increases NO and ROS production in BV-2 and primary murine microglia. a The cellular redox status was determined using carboxy-H2DCFDA. Serum-starved BV-2 cells and PMM were incubated with carboxy-H2DCFDA and treated with LPA (1 μM), and the fluorescence intensity was quantitated. tert-Butyl hydroperoxide (TBHP) was used to induce intracellular ROS formation. Results (four independent experiments performed in triplicate) are expressed as mean values + SD. (**p ≤ 0.01; ***p < 0.001; one-way ANOVA with Bonferroni correction). b Serum-starved cells were treated with LPA (1 μM) for the indicated time periods. The production of NO was determined by measuring nitrate concentrations. Data (three independent experiments performed in triplicate) are presented as mean values + SD. (*p < 0.05; **p < 0.01; one-way ANOVA with Bonferroni correction)

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