Fig. 5

NRG-1 inhibits the degradation of IkB-α in LPS-stimulated N9 microglia cells. N9 microglial cells were pre-treated with 100 ng/ml NRG-1 for 24 h followed by the absence or presence of LPS (10 μg/ml) for 1 h (a) and 3 h (b). Whole cell extracts were taken from untreated cells or cells pre-treated NRG-1 alone or the absence or presence of LPS (10 μg/ml) and were assayed using western blot. The band intensity was quantified using studio lite imager and is presented relative to the level of β-actin. Data are presented for three independent experiments. Results were expressed as the mean +/− SD. Asterisk denotes significant difference compared to cells treated with only LPS (p < 0.05)