Fig. 4
Gly injection did not reduce infarction in SCID mice and T cell-deficient nude rats. Infarct size in the ipsilateral hemisphere was measured 3 days after stroke onset using TTC staining, then normalized to the contralateral hemisphere and expressed as a percentage. a The effects of Gly injection on brain infarction in SCID mice with and without T cell reconstitution. n = 7 to 9/each group. *P < 0.05 between the two indicated groups. b Gly treatment did not reduce infarct size in focal ischemia with 100 min MCA suture occlusion in nude rats. The drug was injected i.p. immediately before stroke onset and 2 h after reperfusion. The animals were divided into four groups. Nude rats in the first two groups were injected with vehicle and Gly, while nude rats in the second two groups were reconstituted with wild-type splenocytes, thus T cells were reconstituted. ***P < 0.001 vs control ischemia with vehicle injection; # P < 0.05 between the two indicated groups. c The effects of Gly on neuronal death in the co-culture with splenocytes. Neurons were co-cultured with wild-type (WT) and SCID splenocytes. *P < 0.05 between the two indicated groups