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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Infliximab reduces peripheral inflammation, neuroinflammation, and extracellular GABA in the cerebellum and improves learning and motor coordination in rats with hepatic encephalopathy

Fig. 7

Altered membrane expression of GABA transporters in the cerebellum of PCS rats. Membrane expression of GAT3 (a) and GAT1 (b) was analyzed using the BS3 crosslinker procedure as described in the “Methods” section. Samples, incubated in the absence or presence of BS3, were subjected to western blotting using antibodies against each transporter. Representative images are shown. Samples in the absence of BS3 represent the total amount of each protein. Samples in the presence of BS3 represent the non-membrane fraction. The intensities of the bands were quantified and membrane expression was calculated as the difference of intensity between samples without and with BS3. Values are expressed as percentage of control rats and are the mean ± SEM of 8 rats per group for GAT1; 6 sham rats treated with vehicle and 7 sham rats treated with infliximab; and 6 PCS rats treated with vehicle and 5 PCS rats treated with infliximab for GAT3. Values significantly different from control rats are indicated by asterisks and from PCS rats by a. * p < 0.05; a p < 0.05

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Journal of Neuroinflammation

ISSN: 1742-2094