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Table 3 Primer sequences for the 3′-UTR of SIRT1

From: miR-124a and miR-155 enhance differentiation of regulatory T cells in patients with neuropathic pain

Primer

Sequence

Restriction site

SIRT1-3′UTR for

5′-CTCGAGCTGTGAAACAGGAAGTAACAGACA-3′

XhoI

SIRT1-3′UTR rev

5′-GTTTAAA CTGGCAGTAATGGTCCTAGCTG-3′

PmeI

  1. Restriction enzymes and the cutting sides of these enzymes are italicized