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Table 4 Primers for mutagenesis

From: miR-124a and miR-155 enhance differentiation of regulatory T cells in patients with neuropathic pain

Primer Sequence Position
SIRT mut 124a for 5′-TATTTAAAAGCTTAGCCTGGATTAAAACTAGAGATCAACTTTCTCAGA-3′ 1211–1217
SIRT mut 124a rev 5′-GCTGAGAAAGTTGATCTCTAGTTTTAATCCAGGCTAAGCTTTTAAATA-3′  
SIRT mut 155_1 for 5′-CAGGAATTGTTCCACCAGGGTTAGGAACTTTAGCATGTC-3′ 36–42
SIRT mut 155_1 rev 5′-GACATGCTAAAGTTCCTAACCCTGGTGGAACAATTCCTG-3′  
SIRT mut 155_2 for 5′-TTGATCTTTTCCACAAGGGTTAAACTGCCAAAATGTG-3′ 929–935
SIRT mut 155_2 rev 5′-CACATTTTGGCAGTTTAACCCTTGTGGAAAAGATCAA-3′  
SIRT mut 155_3 for 5′-GAAATTGCACAGTAAGGGTTTATTTTTCAGACCATT-3′ 1408–1414
SIRT mut 155_3 rev 5′-AATGGTCTGAAAAATAAACCCTTACTGTGCAATTTC-3′  
  1. Sequenzes of the mutagenesis are italicized