Fig. 2
From: Interferon-γ blocks signalling through PDGFRβ in human brain pericytes
Human brain pericytes respond to PDGF-BB signals in vitro. a Pericytes were serum starved for 2 h, then treated with vehicle (lanes 1 and 7) or PDGF-BB (100 ng/mL) (lanes 2–6, and 8) for the indicated times, and analysed by SDS-PAGE. Representative blots from three cases are shown. b Blots from a were analysed and quantified with Image Studio™. Phosphorylated PDGFRβ (Tyr751) (p-PDGFRβ) (b), PDGFRβ (c), and PDGF-BB (f) were normalized to GAPDH; phosphorylated Akt (Ser473) (p-Akt) (d) and phosphorylated ERK (Tyr204) (p-ERK) (e) were normalized to total Akt and ERK, respectively. **Bottom band in pERK blot is GAPDH