Table 1 Antibodies and dilutions used in this study
From: Interferon-γ blocks signalling through PDGFRβ in human brain pericytes
Company | Catalogue number | WB | ICC | |
|---|---|---|---|---|
Primary antibodies | ||||
PDGFRβ rabbit monoclonal | Cell Signaling | 3169 | 1/1000 | 1/500 |
Phospho-PDGFRβ Tyr 751 rabbit monoclonal | Cell Signaling | C63G6 | 1/1000 | |
PDGFRβ rabbit monoclonal | Abcam | 35270 | 1/500 | |
αSMA mouse monoclonal | Dako | IS611 | 1/10 | 1/4 |
Akt mouse monoclonal | Cell Signaling | 2920 | 1/2000 | |
Phospho-Akt S473 rabbit polyclonal | Cell Signaling | 9271 | 1/1000 | |
Phospho-ERK mouse monoclonal | Santa Cruz | sc-7383 | 1/500 | |
PDGF-BB rabbit polyclonal | Abcam | ab23914 | 1/800 | |
ERK 1 (K-23) rabbit polyclonal | Santa Cruz | sc-94 rabbit | 1/500 | |
GAPDH mouse monoclonal | Abcam | ab9484 | 1/1500 | |
CD140b (PDGFRβ-N-terminal) | AbD Serotec | 7460-3104 | 1/200 | |
Ki67 rabbit polyclonal | Dako | A0047 | 1/500 | |
aCD140b-PE | BD Biosciences | 558821 | 1/5 | |
Secondary antibodies | ||||
Goat anti-rabbit Alexa 488 | Life Technologies | A11034 | 1/500 | |
Goat anti-mouse Alexa 594 | Life Technologies | A11032 | 1/500 | |
Goat anti-mouse IRDye-680LT | LiCOR | 926-68020 | 1/10000 | |
Goat anti-rabbit IRDye-800CW | LiCOR | 926-32211 | 1/10000 | |