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Table 2 Excitation and emission parameters for ImageXpress micro XLS™ high content screening system

From: Interferon-γ blocks signalling through PDGFRβ in human brain pericytes

Cube

Filters

Lumencor light engine

Ex range

Ex peak

Dichroic

Em range

Em peak

Triple 4

DAPI

UV (380–410)

381–399

390/18

436

445–469

457

Triple 4

FITC

Cyan (460–490)

484–504

494/20

514

518–542

530

Triple 4

TRED

Green (535–600)

561–590

575/25

604

612–643

628

Quad 5

DAPI

UV (380–410)

381–399

390/18

410

419–460

440

Quad 5

FITC

Cyan (460–490)

474–496

485/20

504

507/533

521

Quad 5

CY5

Red (620–750)

644–656

650/13

669

675–723

700

  1. Microscope information for all image acquisition in this study including light source, filters, excitation, and emission range and peaks in nanometer