Table 3 The list of primers used for qRT-PCR in this study
From: Interferon-γ blocks signalling through PDGFRβ in human brain pericytes
Accession number | Gene | Sequence | Amplicon length | |
|---|---|---|---|---|
NM_002046.4 | GAPDH (h) | Fw | CATGAGAAGTATGACAACAGCCT | 113 bp |
Rv | AGTCCTTCCACGATACCAAAGT | |||
NM_001565.3 | IP-10 (CXCL10) | Fw | TGGCACACTAGCCCCACGTT | 88 bp |
Rv | TGCTGAGACTGGAGGTTCCTCTGC | |||
NM_002982.3 | MCP-1 (CCL2) | Fw | CAGCCAGATGCAATCAATGCC | 190 bp |
Rv | TGGAATCCTGAACCCACTTCT | |||
NM_000963.2 | COX2 (PTGS2) | Fw | AGGGTTGCTGGTGGTAGGAA | 76 bp |
Rv | TCTGCCTGCTCTGGTCAATG | |||
NM_000201.2 | ICAM1 (h) | Fw | GAACCAGAGCCAGGAGACAC | 84 bp |
Rv | GAGACCTCTGGCTTCGTCAG | |||
NM_001025159.2 | CD74 | Fw | GAGTCACTGGAACTGGAGGAC | 81 bp |
Rv | CTGCTCTCACATGGGGACTG | |||
NM_002609.3 | PDGFRβ | Fw | CGCAAAGAAAGTGGGCGGCT | 101 bp |
Rv | TGCAGGATGGAGCGGATGTGGT | |||
NM_001141945.2 | αSMA | Fw | ACGTGGGTGACGAAGCACAGA | 84 bp |
Rv | CGTCCCAGTTGGTGATGATGCC | |||
NM_001145966.1 | Ki67 | Fw | AGCGGAAGCTGGACGCAGAA | 79 bp |
Rv | TCCAGGGGTTGGGCCTTTTCCT |