Fig. 2
Ca2+ channel blockers MK-801 and nimodipine abrogate neurotoxicity of CXCL12 in cerebrocortical cell cultures. Incubation with CXCL12 and Ca2+ channel blockers and assessment of neuronal survival was performed as described in the text using staining for neurons (MAP-2) and nuclear DNA. Representative images are shown for six experimental conditions (MK-801 at 10 μM). Scale bar, 20 μm. Values in the graph are mean ± SEM; n ≥ 3 with duplicate or triplicate samples per condition; *P ≤ 0.05, ***P ≤ 0.001 by ANOVA followed by Fisher’s PLSD post hoc test