Fig. 2

PARP inhibition decreases leukocyte adhesion in brain microvessels during systemic inflammation. a Representative images from videos of leukocytes labeled with calcein-AM. b Measurements of leukocyte firm (not rolling) adhesion during a 30-s observation period (ex vivo treated, calcein-AM-labeled leukocytes). Leukocytes were treated ex vivo with or without PARP inhibitors for 4 h. c Measurements of leukocyte firm (not rolling) adhesion during a 30-s observation period (autologous, Rhodamine 6G-labeled leukocytes). Results are shown as mean adhesion ± SEM (four to six animals per treatment group). ns indicates no significance, **P < 0.01, ***P < 0.001 indicates significance vs. NT. Scale bar = 100 μm is shown in one micrograph, but it is valid for all micrographs shown