Fig. 3

PARP ko mice show diminution of leukocyte adhesion to the brain endothelium in vivo. a Representative images from videos of leukocytes labeled with calcein-AM (upper panels) or Rhodamine 6G (lower panels). b Measurement of labeled leukocytes under firm (not rolling) adhesion in WT or PARP ko mice during a 30-s observation period (ex vivo treated, calcein-AM-labeled leukocytes). c Measurements of leukocyte firm (not rolling) adhesion during a 30-s observation period (autologous, Rhodamine-labeled leukocytes). Results are shown as mean adhesion ± SEM (four animals per treatment group). ns indicates no significance, **P < 0.01, ***P < 0.001, ****P < 0.0001 indicates significance vs. LPS treated. Scale bar = 100 μm is shown in one micrograph, but it is valid for all micrographs shown