Fig. 1
Effects of EA on A20 expression in the rat brain after I/R. a A20 mRNA levels in the rat brain were measured at the indicated time points using RT-qPCR. The graph shows the relative mRNA levels after normalization to the housekeeping gene β-actin. Data are presented as means ± SEM; n = 5 per time point per group. ***P < 0.001 vs. the MCAO group; ### P < 0.001 vs. the sham group. b Western blots showing the expression of A20 in rat brains at the indicated time points. The housekeeping protein β-actin was used as a loading control. c Graph showing the relative A20 protein levels after normalized to β-actin. Data are presented as means ± SEM; n = 3 per time point per group. **P < 0.01, ***P < 0.001 vs. the MCAO group; # P < 0.05, ### P < 0.001 vs. the sham group. d Immunofluorescence staining of A20 expression in the ischemia/reperfusion cortex of MCAO and MCAO + EA group rats 24 h after reperfusion and in the cortex of the sham group rats. Scale bar = 50 μm. e The A20-positive cell counts in the ischemia/reperfusion cortex of MCAO and MCAO + EA rats and the cortex of rats in the sham group. The A20-positive cell counts were expressed as number/in.2. Data are presented as means ± SEM; n = 5 per group. ### P < 0.001 vs. the sham group; ***P < 0.001 vs. the MCAO group