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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats

Fig. 7

EA suppressed NF-kB signaling by upregulating A20. a Western blots showing the expression of A20, p-IKKβ, IKKβ, p-IkBα, IkBα, nuclear p65, and cytoplasmic p65 in ischemia/reperfusion cortical extracts 24 h after reperfusion. β-actin was used as the loading controls for total and cytoplasm proteins, and lamin B was used as the nuclear loading control. b The phosphorylation ratio of IKKβ and IkBα is presented as the p-IKKβ/IKKβ and p-IkBα/IkBα ratios, respectively. The nuclear translocation ratio of NF-kB p65 was indicated as nucleus-p65/cytoplasm-p65. n = 5 per group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the MCAO group; ### P < 0.001 vs. the MCAO + EA group. c At 24 h after reperfusion, NF-kB p65 immunohistochemistry (brown) was performed to show NF-kB p65 nuclear translocation in the ischemia/reperfusion cortex of rats from the four groups (sham, MCAO, MCAO + EA, MCAO + EA + LV-shA20, n = 5 per group). Yellow arrows indicate NF-kB p65 mainly in cytoplasm, and red arrows indicate NF-kB p65 both in cytoplasm and nucleus. Scale bar = 50 μm. d Immunofluorescence staining was performed to demonstrate NF-kB p65 expression (red) in neurons (green) of ischemia/reperfusion cortex of rats from the four groups (sham, MCAO, MCAO + EA, MCAO + EA + LV-shA20, n = 5 per group) at reperfusion 24 h. Yellow arrows indicate NF-kB p65 mainly in cytoplasm, and red arrows indicate NF-kB p65 both in cytoplasm and nucleus. Scale bar = 50 μm

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