Fig. 1

MMF rescues forebrain neurons from OGD-induced cell death in culture. Cultures of E15 rat forebrain were treated with MMF (25, 50, or 100 μM) beginning 12 h prior to OGD deprivation and throughout the remaining culture period. Cultures treated with MMF contained significantly fewer dead/dying cells at 24 h (a–d, i) and 48 h (e–h) than untreated controls as assessed by LIVE/DEAD assay (a–i, k) or assay of LDH released into the media by dead cells (j). *p < 0.05; **p < 0.01, Student’s t test