Fig. 2
From: EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis
SS2 strain SC19 induces tyrosine phosphorylation of several cellular proteins. a Tyrosine phosphorylation of the hBMEC extracts in response to SS2 infection were analyzed by immunoblotting with anti-phosphotyrosine antibody (PY). The tyrosine-phosphorylation of several proteins could be markedly inhibited by AG1478. β-actin was analyzed as a normalization control. b The hBMEC was lysed and subjected to immunoprecipitation with an anti-EGFR antibody and immunoblotting with the anti-phosphotyrosine mAb 4G10. c Bacteria were cultured in TSB containing 10 % newborn bovine serum, and treatment with 5 M AG1478 or vehicle (DMSO) had no influence on bacterial growth at 37 °C. d Treatment with 5 μM AG1478 did not influence the hBMEC proliferation, as demonstrated by MTT assay