Fig. 10
From: Adoptive transfer of M2 macrophages reduces neuropathic pain via opioid peptides
Analysis of CD163 and MHC II expression in perineurally injected CFSE-labeled polarized macrophages in injured nerves. Injected macrophages were identified as F4/80+CFSE+ cells. a Percentage of injected macrophages expressing CD163 following one injection of DMEM only or CFSE-labeled M0, M1, and M2 cells. b Percentage of injected macrophages expressing CD163 following one injections of DMEM only or CFSE-labeled M0, M1, and M2 cells. c Percentage of injected macrophages expressing MHC II following one injection of DMEM only or CFSE-labeled M0, M1, and M2 cells. d Percentage of injected macrophages expressing MHC II following two injections of DMEM only or CFSE-labeled M0, M1, and M2 cells. In all experiments, DMEM medium or CFSE-labeled macrophages were injected at the injury site on day 14 (one injection) or on days 14 and 15 (two injections) after CCI. Animals were killed 24 h after the first injection and 24 h after the second injection, and cells from the nerves were analyzed using flow cytometry and FlowJo software. **p < 0.01, ***p < 0.001 (one-way ANOVA, Bonferroni’s test); # p < 0.05 compared to the same treatment 24 h after the first injection (unpaired t test). Data show the mean ± SEM (n = 6 animals per group)