Fig. 9

Analysis of CD163 and MHC II expression in endogenous macrophages in injured nerves following perineural transfer of CFSE-labeled polarized macrophages. Endogenous macrophages were identified as F4/80⁺CFSE⁻ cells. a Percentage of endogenous macrophages expressing CD163 following one injection of DMEM only or CFSE-labeled M0, M1, and M2 cells. b Percentage of endogenous macrophages expressing CD163 following two injections of DMEM only or CFSE-labeled M0, M1, and M2 cells. c Percentage of endogenous macrophages expressing MHC II following one injection of DMEM only or CFSE-labeled M0, M1, and M2 cells. d Percentage of endogenous macrophages expressing MHC II following two injections of DMEM only or CFSE-labeled M0, M1, and M2 cells. In all experiments, DMEM- or CFSE-labeled macrophages were injected at the injury site on day 14 (one injection) or on days 14 and 15 (two injections) after CCI. Animals were killed 24 h after the first injection and 24 h after the second injection, and cells from the nerves were analyzed using flow cytometry and FlowJo software. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA, Bonferroni’s test); ^# p < 0.05, ^## p < 0.01 compared to the same treatment 24 h after the first injection (unpaired t test). Data show the mean ± SEM (n = 6 animals per group)