Fig. 6

Expression of ITPR1 in the spinal cord (a, e–g), sural nerve (h) and dorsal root ganglia (DRG) (b–d, i–j) of adult mouse (a, d), rat (b–c, e–f, i–j) and rhesus monkey (g–h) and as detected by in situ hybridization (a, b, e) and immunostaining (c–d, f–j), and binding of IgG from the patient’s CSF (i) and serum (j) to sensory neuron somata in rat DRG as detected by indirect immunofluorescence. A polyclonal rabbit antibody to ITPR1 (Dianova) and an AlexaFluor®568-labelled goat anti-rabbit IgG secondary antibody were used to detect ITPR1 expression (red). A fluorescein isothiocyanate-labelled goat anti-human IgG antibody was used to visualize bound patient IgG (green). Yellow areas in j indicate overlay of the patient’s IgG and the commercial ITPR1 antibody. a Taken from the Allen Spinal Cord Atlas [64] (http://mousespinal.brain-map.org/). b, c and e Reproduced from Dent et al. [2] with kind permission of the publisher , d from Zhuang et al. [5] (made available under the Creative Commons CC0 public domain dedication; https://creativecommons.org/publicdomain/zero/1.0/) , and f from Sharp et al. [3] with kind permission of the publisher. DH dorsal horn, IP3R inositol-1,4,5 trisphosphate receptor