Fig. 4

Effects of Sino on the activation of astrocytes and microglia, and the expression of DRD2, p-STAT3, and CRYAB in MCAO mice. a–c Double-immunofluorescence staining for neuronal (NeuN), microglial (Iba1), and astrocytic (GFAP) DRD2 expression in the ischemic penumbra area after MCAO. d Double-immunofluorescence staining for astrocytic p-STAT3 and DAPI. The cells indicated with an arrow were magnified. Scale bar = 200 μm. e–j Western blots and quantitative analysis of GFAP, Iba1, DRD2, p-STAT3, and CRYAB expression are expressed as mean ± SEM (n = 5). *p < 0.05 and **p < 0.01 vs. sham group; ^# p < 0.05 between the indicated groups