Fig. 7

The effects of Sino on the nuclear translocation of CRYAB and the activation of STAT3 in astrocytes in vitro. a, b Western blot and quantitative analysis of nuclear CRYAB expression. c–e Immunoprecipitation assay evaluating the binding activity of CRYAB with STAT3 and the phosphorylation of p-STAT3. f, g EMSA assay of the DNA-binding activity of STAT3. Data are expressed as mean ± SEM (n = 5). *p < 0.05 and **p < 0.01 vs. sham group