Fig. 3

DFO reduced neuroinflammation in hippocampus. a Representative bands of western blot for CD68 in hippocampus. b Quantification of CD68 protein level in hippocampus. c Representative pictures of Iba1 staining in hippocampus. d The cell body/cell size ratio of Iba1-labeled microglia. e, f Hippocampal IL-1β and TNF-α. Surgery significantly increased the upregulation of microglia activation, as well as CD68, IL-1β, and TNF-α level in hippocampus. These effects were attenuated by DFO pretreatment. n = 4/group, *P < 0.05, **P < 0.01. Data are expressed as mean ± SD. Scale bar 50 μm. DFO deferoxamine, NS normal saline