Fig. 5

Inhibition of B cell genesis in the bone marrow and impairment of thymocyte development in A. cantonensis-infected mice. a Frequency of B cell progenitors in BM is reduced following infection. Developing B cells (DB) in the BM were gated on B220⁺AA4.1⁺ cells first (upper panels) and further analyzed for IgM⁺CD19⁺ immature (IB) and IgM⁻CD19⁺ pro-/pre-B cell (PB) populations (lower right panels). b Frequency of developing B cell in the spleen is reduced after infection. Mature B cells in the spleen were gated on B220⁺AA4.1⁺ cells. Developing B cells were gated on B220⁺AA4.1⁺ cells first (upper panels) and further analyzed for IgM^hiCD23⁻ TR1, IgM^hiCD23⁺ TR2, and IgM^loCD23⁺ TR3 developing B cells (lower right panels). c Proportion of thymocyte subsets in control and infected mice at 14, 16, 18, and 21 dpi. d Number of thymocyte subsets in control and infected mice at 14, 16, 18, and 21 dpi. e CD4⁺CD8⁺ T cells of thymus were investigated for apoptosis by annexin-V labeling. Each group contained four to six mice. One of three independent experiments with similar results is shown. Values were shown as mean ± SEM. A two-tailed t test was used for statistical analysis. *, results differed from the control group; *P < 0.05; **P < 0.01; ***P < 0.001