Fig. 5

Neuroinflammation induced by in vitro exposure of hippocampal slices to LPS provoked intracellular acidification in CA1. a Representative images of the BCECF-AM-loaded slices treated with saline or LPS taken with the excitation wavelengths 440 and 490 nm and the F ₄₄₀/F ₄₉₀ ratio. ROI used for the pHi measurements are marked by circles. Bar = 100 μm. b Quantification of the data. Averaged pHi levels in the saline- and LPS-treated slices at different post-treatment intervals. Mean ± SEM; saline: n = 37 (2–3.5 h), 14 (3.5–5.5 h), and 21 (5.5–7 h); LPS: n = 18 (2–3.5 h), 22 (3.5–5.5 h), and 14 (5.5–7 h); *p = 0.03, **p = 0.001. c Quantification of the data. The data averaged across the entire visible part of the CA1 Str.Pyr. for the period of 3.5–7 h. Mean ± SEM; saline: n = 72 (saline) and 54 (LPS); ***p = 0.0001