Fig. 2

IFNAR restricts neurotropic flavivirus spread in astrocytes. Primary astrocytes isolated from WT and IFNAR^−/− mice were infected with 0.1 MOI of different flaviviruses. A number of infected cells were determined by immunofluorescence assay staining for DAPI (blue) and flavivirus E protein (red). Scale bar 0.66 mm. a Representative picture of TBEV-infected WT and IFNAR^−/− astrocytes and MEFs (resolution 1024 × 1024) 72 hpi. a, b Hypr European subtype. c Aina, Siberian subtype. d Sofjin Far Eastern subtype. e JEV. f WNV. g ZIKV. Data are cumulative from at least two independent experiments n = 8.*p < 0.05, ***p < 0.001, ****p < 0.0001