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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects

Fig. 3

Astrocytes induce a fast type I IFN response upon TBEV infection which restricts virus replication. Primary WT Astrocytes (a) and MEFs (b) were pretreated with 5000 U IFNαB/D for 16 h before infection with TBEV MOI 0.1. Viral titers were determined by focus forming assay at indicated time points (n = 6). Primary astrocytes and MEFs were infected with TBEV using MOI 0.1. Expression levels of IFN-β (c) and IFNα2 (d) were determined by qPCR (n = 9). Supernatants were collected at the indicated time points, and antiviral activity was determined by VSV-GFP bioassay on MEFs (e, n = 6) or TBEV-based bioassay on WT and IFNAR−/− astrocytes (f, n = 12). g WT and IFNAR−/− astrocytes were treated with either 5000 U IFNαB/D or virus-inactivated supernatant from WT astrocytes 24 hpi (n= 6). TBEV replication was quantified using qPCR and normalized to input viral RNA. Mean values and standard deviations from three independent experiments are shown. *p < 0.05; **p < 0.01; ***p < 0.001

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