Fig. 4

Astrocytes treated with either supernatant or IFNαB/D mount an antiviral response. Primary astrocytes from WT and IFNAR^−/− mice were isolated, and WT cells were either mock treated, treated with IFNαB/D, or treated with inactivated supernatant from TBEV-infected astrocytes. Gene expression was analyzed by deep sequencing. Venn diagram of differentially expressed transcripts in IFNAR^−/−, IFNαB/D, and supernatant-treated astrocytes compared to mock-treated WT astrocytes (fold-change ±2, q value <0.05). Number of totally regulated genes (a), downregulated (b), and upregulated (c). d Overlap of differentially expressed transcripts under all three conditions (n = 112); red color indicates an upregulation whereas blue color correlates with a downregulation. Dynamic range in the heat map is 7.16 to −5.99 log2 fold difference compared to WT untreated astrocytes. Sd standard deviation (n = 3)