Fig. 2

Morphological diversity of CX3CR1^GFP/+ and CCR2^RFP/+ infiltrating Mo/MΦ. a CX3CR1^GFP/+ cells (green) show three different phenotypes at 14 days after MCAo: amoeboid cells (upper left), ramified cells (upper right), and elongated cells located along the vessels (lower left). Blood vessels were immunostained with the endothelial marker GLUT1 (red). Scale bars represent 15 μm. Graph bars represent the frequencies for each described phenotype in the core (C) and periphery (peri-infarct; PI) of the lesion (n = 4 mice/group). b CCR2^RFP/+ cells (red), which are localized in the core of the ischemic lesion (a), have a round shape (arrowheads), while CX3CR1^GFP/+CCR2^RFP/+ double-positive cells (arrows), have an amoeboid or spindle shape with lower level of RFP expression than CCR2^RFP/+ cells (a and b). CX3CR1^GFP/+ cells acquire a ramified phenotype as they localized in the periphery (b and c). Scale bars represent 20 μm