Fig. 3

CX3CR1^GFP/+ Mo/MΦ proliferate in the ischemic brain. a Co-staining of CX3CR1^GFP/+ with the proliferation marker Ki67 shows that some CX3CR1^GFP/+ Mo/MΦ (green) are Ki67 positive (red) 3 and 14 days after MCAo. Scale bars represent 50 μm. b Orthogonal views of CX3CR1^GFP/+ (green) and Ki67+ (red) showing cellular co-localization. On the right, percentage of double-positive CX3CR1^GFP/+/Ki67+ cells over the total CX3CR1^GFP/+ cells in the ischemic brain 3 and 14 days after MCAo (n = 4 mice/group). c Ki67+ cells do not co-localized with CCR2^RFP/+ cells in the ischemic region 3 days after MCAo