Fig. 5

Ly6C^hi and Ly6C^lo Mo/MΦ do not accumulate in the brain of CCR2^−/− mice after MCAo. a Flow cytometry analysis of infiltrating Mo/MΦ, identified by high expression of CD45 and CD11b and low expression of Ly6G (CD45^hiCD11b⁺Ly6G⁻), shows an increase of Ly6C^hi Mo/MΦ as compared with Ly6C^lo Mo/MΦ in the wild type (WT) mouse 3 days after MCAo. The accumulation of both Ly6C^hi and Ly6C^lo Mo/MΦ is abolished in CCR2^−/− mice. b Brain-resident Ly6C^lo Mo/MΦ outnumbered Ly6C^hi Mo/MΦ in WT mice 14 days after MCAo, while neither Ly6C^hi or Ly6C^lo Mo/MΦ increased in CCR2^−/− mice (n = 4 mice/group). *p < 0.05 vs. WT