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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: N-Docosahexaenoylethanolamine ameliorates LPS-induced neuroinflammation via cAMP/PKA-dependent signaling

Fig. 1

Inhibitory effect of synaptamide on LPS-induced expression of TNF-α and iNOS in microglia cells. Primary rat microglia cells were incubated with LPS (100 ng/mL) and/or synaptamide at the indicated concentration. Synaptamide was added immediately after LPS exposure. Levels of TNF-α (a) and iNOS (b) mRNA were determined by qPCR after 1 h incubation. After 24 h, accumulated levels of TNF-α (c) and nitric oxide (d) in the media were measured by ELISA and Griess reaction. The data represent the mean ± SEM at least three independent experiments. Means designated with the same letter are not significantly different. One-way ANOVA followed by Tukey’s post hoc test (Prism Software, SigmaStat)

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Journal of Neuroinflammation

ISSN: 1742-2094