Fig. 3
From: In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response
miR-155 inhibition affects cytokine signaling machinery. a Left panel: Western blot analysis of phospho-STAT-3, SOCS-1, and SHIP-1 expression in lysates prepared from the cortical tissue of the animals from inhibitor and control groups, at 7 days after dMCAO. Graphs represent WB quantification analysis; Optical density of each band was normalized to β-actin (loading control). The samples were collected from the lesioned hemispheres of five different mice per group. Right panel: higher quantity of the samples described in a was loaded in order to detect and quantify phospho-STAT-3, STAT-3, SOCS-6 (with two corresponding 62 kDa (lmw) and 82 (hmw) bands), and C/EBP-β proteins. Graphs represent quantification of the relative density representing protein expression normalized to actin expression (N = 5–6 animals per group). b, c p-STAT, STAT-3, SOCS-1, SOCS-6, SHIP-1, and C/EBP-β protein expressions were detected and quantified in the samples collected from the lesioned hemispheres of the inhibitor and control animals at 14 (b) and 21 (c) days after dMCAO. N = 5–6 animals per group. At 21 days, the expression levels of SOCS-6 and SHIP-1 were undetectable (not shown). For all graphs: error bars: SEM; Student’s t test, *p < 0.05