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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Appearance of claudin-5+ leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles

Fig. 2

CLN-5+ leukocytes in circulation during early EAE. a Representative z-stack confocal images of PBLs isolated from WT mice at D8 EAE and immunostained with CLN-5 (green isosurface) under non-permeabilized or permeabilized (with Triton X-100) conditions. Leukocytes were obtained a day earlier than in Fig. 1, to ensure those CLN-5+ cells in the circulation had not yet all extravasated. b FACS analysis of leukocytes from D8 EAE mice immunostained with the same antibody shows CLN-5 staining under both non-permeabilized and permeabilized conditions. Leukocytes stained with an isotype antibody were used as control. c Western blot analysis of lysates from the same batch of leukocytes and the same antibody clone used in a and b showing a 23 kDa molecular weight band, consistent with the molecular weight of CLN-5

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