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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Appearance of claudin-5+ leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles

Fig. 3

Appearance of eGFP-CLN-5+ leukocytes in Tie-2-eGFP-CLN-5 mice during EAE. a z-stack confocal images acquired from spinal cord cryosections from Tie-2-eGFP-CLN-5 mice showing distribution of eGFP-CLN-5 (green) in naïve venules and associated with an inflamed venule and perivascular leukocytes at D9 EAE. Inset shows DRAQ staining (blue) highlighting the extent of perivascular cellularity, representing leukocyte infiltrates and a minority fraction of eGFP-CLN-5+ leukocytes. The presence of eGFP-CLN-5+ leukocytes in the CNS is consistent with these cells having acquired eGFP-CLN-5 from endothelial sources. b PBLs were isolated from Tie-2-eGFP-CLN-5 mice (n = 5) at D8 post-EAE induction and then subjected to FACS analysis and percentage eGFP-CLN-5+ CD45+ recorded (right). Control PBLs from naïve, WT mice were used to set the gate (left)

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