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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Appearance of claudin-5+ leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles

Fig. 7

EV-like structures in situ at sites proximal to leukocyte adhesion. Serial EM images from mouse spinal cord sections obtained at D13 EAE. a Single serial section showing cross section of an inflamed venule highlighting adherent leukocytes, some apparently undergoing TEM. Inset highlights EV-like membrane-bound structures (blue arrowhead) at the leukocyte-endothelial interface of an adherent leukocyte (blue box). b Representative 3D reconstruction of an adherent leukocyte shown in a, generated from 130 serial slices. The larger membranous structure indicated by * is on the order of 1 μm in diameter and may represent an apoptotic body loosely tethered to the leukocyte. c Contour surface reconstruction of the “traced” leukocyte (red), EV-like structures (green), and endothelium (turquoise) in all 130 serial slices, providing a 3D view of all three elements at the site of leukocyte docking (yellow asterisk). Inset provides an oblique view of the site of leukocyte attachment, showing EV-like structures and leukocyte process (yellow arrowheads) apparently below the surface of the endothelial plasma membrane. d, e 3D surface reconstruction of the leukocyte in panels ac, along with a representative serial EM slice, highlighting various patterns and sizes of EV-like structures, close to the site of leukocyte binding

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