Fig. 4

Quantification of microglia and myelin coverage following injection of BFP-MSC or IL13-MSC on the right side of the splenium in CPZ-treated mice. a Representative overview images of the splenium of the corpus callosum with CX₃CR1⁺ microglia (green) and MBP⁺ myelin (purple) in control, CPZ-treated mice and CPZ-treated mice with BFP-MSC grafts or IL13-MSC grafts. The areas which were analysed are delineated by a dotted line. Scale bar indicates 200 μm. b Dot plots showing the coverage percentage of eGFP in the whole splenium (left graph), the right splenium (middle graph) and the left splenium (right graph) for each experimental group. c Dot plots showing the coverage percentage of MBP in the whole splenium (left graph), the right splenium (middle graph) and the left splenium (right graph) for each experimental group. The mean of each independent experiment is represented by a horizontal line. Significant differences are indicated with one (p value <0.05), two (p value ≤ 0.01) or three (p value ≤ 0.001) asterisks. d Dot plots showing the correlation between eGFP and MBP coverage percentage (left graph), eGFP coverage percentage and T ₂ relaxation times (middle graph), MBP coverage percentage and T ₂ relaxation times (right graph) in the splenium for each experimental group. Spearman’s correlation coefficient (r) is presented on each graph. Data points indicated in red on (b and c) correspond with the representative MRI images provided in Fig. 3a, b and with the representative histological images shown in (a)