Immunohistochemistry analysis. Double immunostaining of OX42/CD163, CNPase/CD44 and CD86/NG2 in the lumbar spinal cord of control and EAE rats.
a–d Double labeling for microglial cells marker (OX42) and M2 macrophages marker (CD163) in the white matter of control (a) and EAE experimental groups: 8, 11, and 18 DPI (b–d). Plus in b indicates the activated microglia expressing CD163. e–h Double labeling for oligodendrocyte marker (CNPase) and T lymphocyte marker (CD44) (see also the included high-power magnifications). CD44 is expressed by oligodendrocytes in control. Arrows in e indicate co-localization, although this expression changed at 8 DPI in f. i–l Double labeling for oligodendrocyte precursor cells (NG2) and M1 macrophages marker (CD86). The immunoreactive area of CD163 (m), CNPase (n), CD44 (o), and CD86 (p) in EAE rats compared to controls. Statistical analysis: one-way ANOVA and Dunnett’s multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)