Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons

Fig. 1

Characterization of pluripotency of the LRRK2R1441G mutant iPSC lines (PD-R1 and PD-R4). a, b Reprogrammed cells formed compact uniform colonies that showed robust and uniform expression of typical pluripotent markers such as NANOG and embryonic stage-specific antigen 4 (SSEA-4) by immunofluorescence. Sequence analysis of exon 31 confirmed the presence of the point mutation in the clones selected for this study. c G-band karyotypes for the selected clones. Pluripotency was confirmed d in vitro by embryoid body formation and trilineage differentiation, analyzed by immunofluorescence and e in vivo by formation of teratomas in NOD-SCID mice that showed cells corresponding to the three germ layers. SMA smooth muscle actin, SAct sarcomeric actin, AFP alpha fetoprotein, TUJ1 βIII-tubulin, TH tyrosine hydroxylase, H/E hematoxylin/eosin. Scale bars are indicated in each panel

Back to article page