Fig. 5
From: Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons
Effect of TNFα on α-synuclein. a Western blot analysis showing α-synuclein and b p62 proteins in mature neurons after treatment with TNFα, at 2 and 6 h. Proteins were resolved in 15% SDS polyacrylamide gels for the visualization of different α-synuclein oligomers. The band at 15 kDa corresponds to the monomer. A band at 50 kDa (asterisk) could correspond to an oligomeric form of α-synuclein. α-tubulin was used as loading control. Statistical analysis showed a significant effect of time on α-synuclein monomer levels in three to six independent experiments, including two different cell lines per genotype (RT-ANOVA, effect of time, P = 0.035). c Control WT neurons were stimulated with TNFα and with IL-1β to evaluate the interaction between p62 and α-synuclein. Co-immunoprecipitation of p62 and α-synuclein using specific antibodies did not show any association under these conditions. Sam68 was used as the loading control. NS non-stimulated, IP immunoprecipitation, FT flow-through. See Additional file 4 for uncropped blots