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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Inhibition of STAT3- and MAPK-dependent PGE2 synthesis ameliorates phagocytosis of fibrillar β-amyloid peptide (1-42) via EP2 receptor in EMF-stimulated N9 microglial cells

Fig. 1

Flow cytometry and confocal microscopy analyses of the effect of EMF exposure on phagocytic capacity in N9 cells. N9 cells were exposed to 2.45-GHz EMF for 20 min. Untreated cultures were used as sham-exposed controls. N9 cells were subjected to a 1-h process of phagocytosis of fAβ42 with a strong fluorescent signal label HiLyte™ Fluor 647 (647-fAβ42) at the indicated time points after EMF exposure. a Continuous engulfment of 647-fAβ42 within 3 h in N9 cells. b Normalized average fluorescence intensity of 647-fAβ42 ingested per group estimated using a flow cytometer and a confocal microscope. *P < 0.05 vs the sham-exposed control group. c Microscopy images of 647-fAβ42 phagocytosis in N9 cells 3 and 12 h after EMF exposure. Scale bar = 20 μm

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